The 16th edition of the annual European Epidermal Barrier Research Network meeting was held in Toulouse, France, from 27th to 28th of September 2018. This event is a meeting point for European scientists that are interested in a broad range of aspects regarding epidermal barrier function. Research topics varied from using CRISPR-Cas9 editing in 3D in vitro diseased skin models and the development of a chicken skin model, to ceramide metabolism and the triple action of Leflunomide, a Rheumatoid Arthritis medicine, for the treatment of inflammatory skin diseases. A poster was presented by Irini Dijkhoff (ESR3) on the build-up of the CITYCARE project and the development of our in-house CITYCARE 3D in vitro skin model. A PDF copy of the poster is available here.